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mpeg1 genes  (Addgene inc)


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    Addgene inc mpeg1 genes
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Mpeg1 Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mpeg1 genes/product/Addgene inc
    Average 93 stars, based on 10 article reviews
    mpeg1 genes - by Bioz Stars, 2026-06
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    Images

    1) Product Images from "Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish ☆ "

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish ☆

    Journal: Current Research in Toxicology

    doi: 10.1016/j.crtox.2025.100276

    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Imaging, Transgenic Assay, Microscopy, Plasmid Preparation



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    Image Search Results


    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Current Research in Toxicology

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    doi: 10.1016/j.crtox.2025.100276

    Figure Lengend Snippet: Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′.

    Techniques: Imaging, Transgenic Assay, Microscopy, Plasmid Preparation